Which technique fractions drugs, hormones, etc., and HbA1c measurement can be done in about 5 minutes?

Which technique fractions drugs, hormones, etc., and HbA1c measurement can be done in about 5 minutes?

• A. Reverse Phase HPLC
• B. High Performance Liquid Chromatography (HPLC)
• C. Mass Spectroscopy
• D. Gas Chromatography/Mass Spectrometry

Answer: (A)

Reverse Phase High-Performance Liquid Chromatography excels at quick, broad separations because it uses a nonpolar stationary phase and a polar mobile phase. By adjusting the gradient, many drugs and hormones can be resolved rapidly, often in about five minutes with fast-column methods and modern equipment. HbA1c measurement can also be done on RP-HPLC platforms with optimized conditions that separate glycated from non-glycated hemoglobin fractions, enabling short run times suitable for routine testing. Mass spectrometry in itself is a detection method and typically requires prior separation; it doesn’t provide the quick fractionation capability alone. Gas chromatography suits volatile or derivatized small molecules and isn’t ideal for large biomolecules like HbA1c, whereas HPLC without specifying RP lacks the well-established fast, broad separations RP methods routinely deliver.

Reverse Phase High-Performance Liquid Chromatography excels at quick, broad separations because it uses a nonpolar stationary phase and a polar mobile phase. By adjusting the gradient, many drugs and hormones can be resolved rapidly, often in about five minutes with fast-column methods and modern equipment. HbA1c measurement can also be done on RP-HPLC platforms with optimized conditions that separate glycated from non-glycated hemoglobin fractions, enabling short run times suitable for routine testing. Mass spectrometry in itself is a detection method and typically requires prior separation; it doesn’t provide the quick fractionation capability alone. Gas chromatography suits volatile or derivatized small molecules and isn’t ideal for large biomolecules like HbA1c, whereas HPLC without specifying RP lacks the well-established fast, broad separations RP methods routinely deliver.