Which method measures antigen-antibody complexes by light scattering?

Which method measures antigen-antibody complexes by light scattering?

• A. Nephelometry
• B. Turbidimetry
• C. Reflectance photometry
• D. Electrophoresis

Answer: (A)

Measuring antigen-antibody complexes through light scattering relies on how those complexes in solution scatter light. When antibodies bind antigens, the formed complexes scatter the incident light, and the amount of scattered light increases with the amount and size of the complexes. Nephelometry specifically detects light that is scattered at an angle away from the incoming beam, which makes it highly sensitive to these complexes. By measuring this scattered light, nephelometry quantifies the antigen-antibody interaction in the sample. The other methods aren’t focused on this angular scattering: turbidimetry looks at the reduction of transmitted light due to scattering, reflectance photometry measures light reflected from a surface, and electrophoresis separates molecules under an electric field. Therefore, the technique that measures antigen-antibody complexes by light scattering is nephelometry.

Measuring antigen-antibody complexes through light scattering relies on how those complexes in solution scatter light. When antibodies bind antigens, the formed complexes scatter the incident light, and the amount of scattered light increases with the amount and size of the complexes. Nephelometry specifically detects light that is scattered at an angle away from the incoming beam, which makes it highly sensitive to these complexes. By measuring this scattered light, nephelometry quantifies the antigen-antibody interaction in the sample. The other methods aren’t focused on this angular scattering: turbidimetry looks at the reduction of transmitted light due to scattering, reflectance photometry measures light reflected from a surface, and electrophoresis separates molecules under an electric field. Therefore, the technique that measures antigen-antibody complexes by light scattering is nephelometry.