Which centrifuge is described as used to obtain a monolayer of cells on slides?

Which centrifuge is described as used to obtain a monolayer of cells on slides?

• A. Cytocentrifuge
• B. Ultra centrifuge
• C. Fixed/Angle-head centrifuge
• D. Horizontal head centrifuge/swinging bucket

Answer: (A)

The idea being tested is how a cytocentrifuge is specifically used to create a thin, uniform layer of cells on a slide for microscopic evaluation. This instrument uses a small-volume, slide-mounted chamber that sits in the rotor. When the sample is spun at relatively gentle, targeted speeds, cells are deposited onto a defined area of the slide rather than forming a thick pellet. The result is a monolayer—a single, even layer of cells—whose morphology is preserved well enough for staining and detailed examination under the microscope. This setup minimizes overlapping and distortion that can occur with thicker smears or pellets, making it ideal for cytology preparations from body fluids, aspirates, and similar samples. Other centrifuges operate at higher speeds, with different head angles, and are designed to pellet material in tubes or form dense pellets, not to deposit a monolayer on a slide, so they don’t achieve the same slide-based thin-layer preparation.

The idea being tested is how a cytocentrifuge is specifically used to create a thin, uniform layer of cells on a slide for microscopic evaluation. This instrument uses a small-volume, slide-mounted chamber that sits in the rotor. When the sample is spun at relatively gentle, targeted speeds, cells are deposited onto a defined area of the slide rather than forming a thick pellet. The result is a monolayer—a single, even layer of cells—whose morphology is preserved well enough for staining and detailed examination under the microscope. This setup minimizes overlapping and distortion that can occur with thicker smears or pellets, making it ideal for cytology preparations from body fluids, aspirates, and similar samples. Other centrifuges operate at higher speeds, with different head angles, and are designed to pellet material in tubes or form dense pellets, not to deposit a monolayer on a slide, so they don’t achieve the same slide-based thin-layer preparation.